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Test Mnemonic:


Specimen Requirements:
Test Included:

Hepatitis B Virus Surface antibody


Routine venipuncture


7mL of blood in SST (serum separator tube).

Minimum Volume:

3 mL of blood


Delivered to Sample Management within 2 hours of collection.     

Specimen Preparation:

Allow specimen to clot completely at room temperature. Centrifuge to separate serum from cells within 2 hours of collection. Transfer serum from collection tube to labeled plastic transport tube. 


Room temperature for ≤ 8 hours,    refrigerated    for ≤ 48 hours, or frozen for indefinitely. (Avoid repeated freeze/thaw cycles).

Causes for Rejection:

Specimens containing particulate matter, specimens containing sodium azide, heat-inactivated specimens, incomplete and/or incorrect sample identification, improper storage/transport, and gross hemolysis and lipemia.

Reference Range:

By report (reports may vary based on instrumentation, patient age and sex)

Turnaround Time:

Monday- Friday: 24 hours


Automated EIA- chemiluminence


Monday-Friday: 7:00 am to 3:30 pm


Special Chemistry

Clinical Indication:

This test is used to evaluate response to immunization and resolution of hepatitis disease. If the patient has no detectable antibody, it is recommended to wait three months before testing again.

Recommended tests to determine acute hepatitis are: Hepatitis A virus antibody IgM, Hepatitis B Surface antigen, Hepatitis B Core antibody IgM and Hepatitis C virus antibody.

Recommended tests to determine chronic hepatitis are: Hepatitis B Surface antigen, Hepatitis B Core antibody IgM and Hepatitis C virus antibody.

CPT 4 Code:

86706 Limited Coverage Test (NCD)


Tests on samples collected less than three months from the initial results will be cancelled, unless approved by a Chemistry Director or their designee. If "indeterminate", the assay is unable to determine if antibody to Hepatitis B surface antigen is present at levels consistent with immunity.  Patient's immune status should be assessed with other clinical information and/or retesting in 4-6 weeks if clinically indicated.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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