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Test Mnemonic:


Specimen Requirements:
Test Included:

Hepatitis B surface antigen


 Serum separator tube (SST) (Preferred), Plasma separator tube (PST) or red stopped vacutainer tube.


Minimum Volume:

3 mL of blood


Delivered to Sample Management within 2 hours of collection.

Specimen Preparation:

Allow specimen to clot completely at room temperature. Centrifuge to separate serum from cells within 2 hours of collection. Transfer serum from collection tube to labeled plastic transport tube. 


Room temperature for ≤ 8 hours, refrigerated for ≤ 48 hours, or frozen for indefinitely.   (Avoid repeated freeze/thaw cycles).       

Causes for Rejection:

Specimens containing particulate matter, specimens containing sodium azide, heat-inactivated specimens, samples from patients on heparin therapy, incomplete and/or incorrect sample identification, improper storage/transport, and gross hemolysis and lipemia.

Reference Range:

By report (reports may vary based on instrumentation, patient age and sex)

Turnaround Time:

Routine: 8 hours, ASAP : 4 hours   


Automated EIA- chemiluminence


24 hours a day; 7 days a week


Clinical Chemistry

Clinical Indication:

This test is suggested for work-up of acute or chronic infections with hepatitis B virus.

Recommended tests to determine acute hepatitis are: Hepatitis A antibody IgM, Hepatitis B surface antigen, Hepatitis B core antibody IgM, and Hepatitis C antibody.

Recommended tests to determine chronic hepatitis are: Hepatitis B surface antigen, Hepatitis B core antibody IgM, and Hepatitis C virus antibody.

CPT 4 Code:

87340 Limited Coverage Test (NCD)



If the patient has previously been positive, it is recommended to wait three months before testing again. If the patient has no detectable antigen, it is recommended to wait two weeks before testing again. Tests on samples collected outside of these parameters will be cancelled unless approved by a Chemistry Director or their designee.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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