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Test Mnemonic:

AC COOX (arterial) or AC VCOOX (venous)

Specimen Requirements:
Test Included:

Total Hemoglobin (THb), Oxygen Saturation (%O2Hb, O2 Sat), Carboxyhemoglobin (%COHb), Methemoglobin (%MetHb), Oxygen Content (Vol% O2).


Especially where measurement of Oxygen Saturation (%O2Hb) is critical, care should be taken to avoid drawing air into the syringe. Bubbles should be removed from the sample immediately.   Volume: 1 mL; Min. Vol: 0.5 mL.


delivered to Sample Management within 10 minutes of collection.

Specimen Preparation:

Keep specimen at room temperature as long as the analysis is completed within 30 minutes of collection

Causes for Rejection:

Clotted; blood leaking from syringe; large air space or bubbles in syringe; syringes with needles attached; and specimens not delivered to the lab within allotted time; incomplete and/or incorrect sample identification, improper storage/transport.                                       

Reference Range:

By report (reports may vary based on instrumentation, patient age and sex).

Turnaround Time:

STAT: 10 minutes; CODE: 5 minutes




Clinical Chemistry High Volume Lab (room 5.134 McCullough), Point-of-Care, ISCU, SICU, MICU, OR, PICU, and TDC4A

CPT 4 Code:



If applicable, state clinical information that is required to be provided with specimen.

Blood must be drawn anaerobically into gas-tight glass or plastic syringes or in vacuum tubes (unless Total Hemoglobin only is requested). If sequential pH/Blood Gas Analysis is to be performed on the same sample, 1000-unit sodium or lithium heparin should be used.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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