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Fungus Direct Prep Exam (088-0019)
Test Mnemonic:


Specimen Requirements:

Aseptic collection technique


Clean container

Minimum Volume:

Visible specimen


Room temperature

Specimen Preparation:

Place sample in clean container


48 hours

Causes for Rejection:

Specimen container unlabeled or labeled incorrectly. No date and time of collection or no collector information on order. Submission of Sputum, CSF, urine, body fluids or blood for this test due to low sensitivity.

Reference Range:

No fungal elements observed

Turnaround Time:

1-3 days


Microscopic examination of specimen with KOH with Calcofluor stain


Clinical Microbiology


KOH Prep; Fungus Prep

Clinical Indication:

Rapid detection of yeast, hyphae and other fungal elements in specimen

Patient Preparation :

Aseptic collection technique

CPT 4 Code:



Requests for fungus cultures of skin scrapings, aspirate, fluid, tissue, hair include  KOH as part of the fungus culture order. Specify this test if on other sample types.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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