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ANTIBODY SCREEN, Indirect (10005502)
Test Mnemonic:


Specimen Requirements:
Test Included:

Indirect antiglobulin testing with screening cells; resolution of a positive antibody screen will require additional testing


Routine venipuncture


Lavender-topped tube (EDTA)

Minimum Volume:

3 mL blood;  additional specimens may be required for antibody identification to resolve a positive test


Ambient temperature on day of collection; refrigerate if delayed


Causes for Rejection:

Specimen improperly labeled, requisition incomplete, serum separator tube, gross hemolysis, frozen sample, failure to identify phlebotomist and verifier


Reference Range:


Turnaround Time:

2 hours if negative; 4 hours or longer if positive




Specimens are accepted 24 hours per day at the Blood Bank lab, 4.404 Clinical Services Wing (CSW).




Indirect Antiglobulin Test; IAT; Alloantibodies; Antibody Screen; Antiglobulin Test, Indirect; Indirect Anti-human Globulin Test; Indirect Coombs; Irregular Antibodies; Unexpected/Atypical/Irregular Antibodies

CPT 4 Code:



All requests must include requesting physician’s name and ID  number, patient’s complete name, UH number, current account number,  patient location, clinical information/diagnosis, identity of phlebotomist and verifier, and date/time of sample collection.   The specimen must be labeled with the patient’s full name and UH number.  Specimens should be transported to the laboratory in a biohazard specimen bag with the request form in the pocket of the bag.  All information must be complete and legible on the specimen and the requisition.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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