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CORD TESTING (10005511, 10005512, 10005507)
Test Mnemonic:


Specimen Requirements:

Testing is generally performed on a cord blood sample collected at the time of birth;  alternatively, a venous or heel stick sample from the infant can be submitted for testing.

Test Included:

Neonatal ABO & Rh;  DAT testing will be performed if indicated based on these results and the maternal history;  eluates are not routinely performed but may be requested by the physician


Cord blood sample from the newborn or heelstick venous sample may be submitted


Lavender-topped tube (EDTA)

Minimum Volume:

3 mL cord blood or 1mL venous sample


Ambient temperature on the day of collection

Causes for Rejection:

Specimen improperly labeled, requisition incomplete, serum separator tube, gross hemolysis, frozen sample, contamination with maternal blood, failure to identify phlebotomist and verifier


Reference Range:

O, A, B, or AB blood group; Rh positive or negative; DAT negative or not required.

Turnaround Time:

2 hours




Specimens are accepted 24 hours per day at the Blood Bank lab, 4.404 Clinical Services Wing (CSW).


Cord Blood Testing, Cord Blood Sample, Neonatal Blood Type and DAT

Clinical Indication:

Testing is indicated when maternal history suggests a potential for HDFN (hemolytic disease of the fetus or newborn) may exist:   maternal blood group O; maternal type Rh negative; maternal antibody screen positive or history of clinically significant antibodies.  Cord blood testing is required for all babies born to Group O and Rh negative mothers as part of the testing process to identify candidates for post-partum Rh Immunoglobulin administration.

CPT 4 Code:

86900, 86901, 86880


All requests must include requesting physician’s name and ID  number, patient’s complete name, UH number, current account number,  patient location, clinical information/diagnosis, identity of phlebotomist and verifier, and date/time of sample collection.   The specimen must be labeled with the patient’s full name and UH number.  Specimens should be transported to the laboratory in a biohazard specimen bag with the request form in the pocket of the bag.  All information must be complete and legible on the specimen and the requisition.  Maternal name and the medical record number will be requested if it is not included on the requisition.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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