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HEMOSIDERIN STAIN (089-8313 (bone marrow);089-0535 (peripheral blood/body fluids))
Test Mnemonic:

RONBM (Bone Marrow); Iron Stain (Peripheral fluids)

Specimen Requirements:

Bone marrow aspirate, CSF, serous fluids, blood. Collect in EDTA. Invert promptly to prevent clotting. CSF- standard sterile tube.


All types of specimens should be delivered to lab promptly. Bone marrow aspirates should be delivered within 30 minutes.

Causes for Rejection:

QNS, old specimen, identification errors, receipt in lab after hours unless prior arrangements made.

Reference Range:

Normal bone marrow is positive for hemosiderin. All body fluids should be negative

Turnaround Time:

 24 hours


 Cytochemical demonstration of iron-bearing pigment (Perl’s Prussian Blue); Microscopy.


 Bone Marrow Aspirate samples are accepted 8AM - 2PM, Monday-Friday, Room 5.142, McCullough. Body fluids and peripheral blood accepted 24 hours.

Clinical Indication:

Classification of anemias, detection of CNS hemorrhage and to evaluate bone marrow iron stores.

CPT 4 Code:



If applicable, state clinical information that is required to be provided with specimen.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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