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Direct Antiglobulin Test (10005505)
Test Mnemonic:


Specimen Requirements:



Test Included:

Direct antiglobulin testing with polyspecific anti-human globulin serum and use of monospecific reagents (anti-IgG, anti-complement) when indicated.  Elution studies and antibody identification testing may be performed as part of the resolution for positive Direct Antiglobulin Test results.


Routine venipuncture


Lavender-topped tube (EDTA)

Minimum Volume:

3mL blood


Ambient temperature

Causes for Rejection:

Specimen improperly labeled, requisition incomplete, serum separator tube used, gross hemolysis, frozen sample, failure to identify phlebotomist and verifier.

Reference Range:


Turnaround Time:

2 hours if negative;  positive tests may take multiple hours for resolution


Direct agglutination of patient cells with reagent antisera


Specimens are accepted 24 hours per day at the Blood Bank lab, 4.404 Clinical Services Wing (CSW).


Antiglobulin Test, Direct; Anti-human Globulin Test, Direct; Anti-human Serum Test, Direct; DAT; Direct Coombs; DAT

CPT 4 Code:



Diagnosis, history of transfusions at other hospitals, and list of medications received by the patient during this admission and for 3 months prior to admission should be included with the requisition.

All requests must include the requesting physician's name and ID number, the patient's complete name, UH number, current account number, patient location, clinical diagnosis, identity of phlebotomist and verifier, and the date/time of sample collection.  The specimen must be labeled with the patient's full name and UH number.  Specimens should be transported to the laboratory in a biohazard specimen bag with the request form in the pocket of the bag.  All information must be complete and legible on the specimen and requisition.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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