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Reptilase Time (089-0514)
Test Mnemonic:

 REPTILASE

Specimen Requirements:
Collection:

Blue-topped (3.2% sodium-citrate) tube. Routine venipuncture; discard 1st mL of blood by collecting a discard tube prior to collecting the blue-topped (3.2% sodium-citrate) tube.  For collections with butterfly blood collection sets, a discard tube should also be collected prior to collection of the blue top to ensure sufficient sample volume. Drawing a discard tube will displace the air from the blood collection set tubing to ensure proper blood draw volume.

Container:

Blue-topped (3.2% sodium-citrate) tube

Storage/Transport:

 Separate the plasma from the cells and transport at 20 +/- 5 0C

Specimen Preparation:

Centrifuge the sample 15 minutes at 2500g.

Stability:

Whole blood 4 hours at room temperature. Plasma storage 8 hours at 20+/- 5°C.  

Causes for Rejection:

QNS, clotted, severely hemolyzed specimen, plasma specimen greater than 8 hours old, wrong tube, high hematocrit (> 55%), sample identification error, sample processed/transported, and/or stored improperly.

Reference Range:

13.6 - 19.2  seconds

Turnaround Time:

  STAT: 1 hour; ROUTINE: 4 hours

Methodology:

 Electromagnetic Viscosity Detection

Performed:

Samples are accepted 24 hours per day at Sample Management, 7.412 CSW Bldg.

Clinical Indication:

Useful in differentiating causes of prolonged thrombin times

CPT 4 Code:

85635

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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