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Lymphocyte Crossmatch (082-0039,082-0115(Renal); 082-0057(Non-Renal); 082-0009,082-0117(Deceased donor,Renal); 082-0062,082-0119(Deceased donor,Non-Renal))
Test Mnemonic:

T22,TAHG,TDTT,B22,BDTT,XMX T22, XMX TAHG, XMX TDTT, XMX B22, XMX BDTT, FLOW, FLOWD, FLOWD X

Specimen Requirements:
Collection:

Blood as follows:

From PATIENT: 1x 10 mL red-top tube

From DONOR: 5x 10 mL pale yellow-top (acid citrate dextrose) tubes and 1x 10 mL red-top tube

From DECEASED DONOR: 1x 10 mL red-top tube AND 5x 10 mL pale yellow-top (acid citrate dextrose) tubes (Spleen segment or lymph nodes may be used in place of blood)

Storage/Transport:

Room Temperature; do not refrigerate

Specimen Preparation:

Routine venipuncture

Stability:

Deliver immediately to Tissue Antigen Lab

Causes for Rejection:

Sample more than 72 hours old, incorrect tube, incorrect label, insufficient white cell count, poor viability

Reference Range:

N/A

Turnaround Time:

Routine: 4 days   STAT: 8 hours

Methodology:

T & B Cell, allogeneic and/or autogeneic XM'S - Flow Cytometry and/or Cytotoxic

Performed:

T Cell Crossmatch (XM), B Cell XM, Auto XM, Flow Cytometry XM

Lab:

Tissue Antigen Laboratory

Synonyms:

Cytotoxic Crossmatch, Flow Cytometry Crossmatch

Clinical Indication:

Transplant Candidate

Patient Preparation :

Predialysis (if applicable)

CPT 4 Code:

86805(Cytotoxic XM), 86825, 56826(Flow Cytometry XM)

Note:

Samples can be delivered to the Tissue Antigen Laboratory, Room 3.350J, Children's Hospital, Monday-Friday, 8 AM to 5:00 PM. Samples received after noon will be processed the next business day.

Schedule 24 hours in advance. The request form must contain the following information: patient name, UH #, physician's name, route, and extension.

When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent.
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