| Cytomegalovirus (CMV) DNA, Real-Time PCR Assay (138-7497) | |
|---|---|
| Test Mnemonic: | CMV PCR |
| Specimen Requirements: | |
| Collection: | Blood - PPT (white top) or gold (SST) tube. Urine, CSF and other sample types, collect in a sterile container. |
| Container: | PPT for blood, sterile container for other sample types |
| Minimum Volume: | 4ml |
| Storage/Transport: | Serum, plasma and other sample types - store frozen. Whole blood - store refrigerated. |
| Specimen Preparation: | Within six hours of collection, centrifuge PPT or SST tube at 1000g for 15 minutes. After centrifugation, remove plasma or serum from cells into a screw-top polyproylene transport tube. Label tube as serum or plasmsa. Do not centrifuge whole blood or bone marrow samples. No specimen prep required for urine or other sample types |
| Stability: | 24 hours |
| Causes for Rejection: | Incomplete and/or incorrect sample identification, insufficient sample collection, gross hemolysis, clotted samples, incorrect tube type, non-sterile or leaking containers |
| Reference Range: | <200 copies/mL |
| Turnaround Time: | 3-7 days, please call lab at (409)772-4197 if quicker turnaround time is needed |
| Methodology: | Real-time PCR |
| Performed: | Molecular Diagnostics Laboratory |
| Synonyms: | CMV Quantitative viral load, Cytomegalovirus viral load; CMV Viral Load; CMV PCR test |
| Clinical Indication: | To monitor active CMV infections |
| Patient Preparation : | Plasma, serum, urine, CSF and BAL samples have been validated and are accepted for testing. Contact laboratory at (409)772-4197 for approval or testing of other sample types when there is a critical need for this assay. (6/25/2014) |
| CPT 4 Code: | 87497 |
| Note: | Submit one PPT tube for each molecular assay requested due to volume needs for testing. This assay measures cytomegalovirus (CMV) DNA using real-time PCR. The dynamic range of the assay is 200-100,000,000 copies per ml. A result of <200 copies/mL does not rule out the presence of PCR inhibitors in the patient's specimen or that CMV virus DNA concentrations may be below the level of quantification. Care should be taken when interpreting any single viral load determination and the test is intended for use in conjunction with clinical presentation and other laboratory results. |
| When ordering tests for which Medicare or Medicaid reimbursement will be sought, physicians should only order tests that are medically necessary for the diagnosis or treatment of the patient. Components of the organ or disease panels may be ordered individually. The diagnostic information must substantiate all tests ordered and must be in the form of an ICD-10 code or its verbal equivalent. | |