Biorepository and Mass Spectrometry Laboratory


The Biorepository and Mass Spectrometry Laboratory (~3,000 sq ft space) is located on the 10th floor of the Medical Research Building. It has a satellite laboratory (approximately 300 sq ft) for the initial processing of human samples located on the 4th floor of the John Sealy Annex Building. The inter-connecting labs in the MRB allow for science collaboration among researchers interested in muscle biology and metabolism in aging.

Contact

The Laboratory is directed by Dr. Elena Volpi.

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Analytical Services

  • Sample biorepository and tracking
  • Measurement of stable isotope enrichment in biological samples by mass spectrometry (protein, lipid and glucose metabolism)
  • Metabolic modeling

Equipment

  • one Agilent Technologies 6890 Gas Chromatograph 5973N Mass Spectrometers with EI and autosampler
  • one Agilent Technologies 6890N Gas Chromatograph 5973N Mass Spectrometers with EI/PCI/NCI bundle and autosampler
  • one Agilent Technologies 6890N Gas Chromatograph 5975B Mass Spectrometers with EI and autosampler
PepperWetLab
  • two Agilent Technologies 6890A Gas Chromatograph 5975C Mass Spectrometers with EI and autosampler
  • one Agilent Technologies 6890 Gas Chromatograph equipped with Flame Ionization Detector and autosampler
  • one Thermo Delta V Gas Chromatograph-Combustion-Isotope Ratio Mass Spectrometer with GasBench for measurement of very low enrichments of deuterium, 13C, 15N, 18O, in muscle, blood and breath
  • three Speed-vacuum systems for sample concentration and desiccation
  • one nitrogen evaporator (N-Evap) for sample desiccation
  • one Sorvall microcentrifuge
  • one Jouan bench centrifuge to separate blood subfractions
  • one Spectrophotometer
  • one MiniProtean electrophoresis system with blotting apparatus
  • nine -80° C freezers
  • one -20° C freezer
  • two 4 C° refrigerator for sample and reagent storage
  • one 121 L liquid nitrogen dewar for tissue sample storage
  • one Biosafety sterile cabinet
  • four fume hoods
  • standard lab items (e.g. water-baths, microplate reader, rotators, shakers, sonicators, high-speed and micro centrifuges)