Older libraries PCR protocol
Jan 14, 2021,
12:02 PM
by
Guy Nir
C01beta NonHOPs library:
|
Reagent
|
Initial
|
Final
|
1 reaction (μl)
|
| Kapa Buffer A |
10x |
1x |
10 |
| Forward |
10 uM |
0.4 uM |
4 |
| Reverse (T7) |
10 uM |
0.4 uM |
4 |
| dNTP mix |
10 mM |
0.4 mM |
4 |
| Kapa Taq |
|
|
1 |
| Raw library |
0.2 ng/ul |
1µl per 10µl of reaction |
10 |
| Water (up to 100 ul) |
|
|
67 |
C01beta HOPs library and HOPs1 of HOPs_12_49 library:
|
Reagent
|
Initial
|
Final
|
1 reaction (μl)
|
| Kapa Buffer A |
10x |
1x |
10 |
| Forward |
10 uM |
0.4 uM |
8 |
| Reverse (T7) |
10 uM |
0.4 uM |
8 |
| dNTP mix |
10 mM |
4 |
| Kapa Taq |
|
|
1 |
| Raw library |
2 ng/ul |
1µl per 10µl of reaction |
10 |
| Water (up to 100 ul) |
|
|
59 |
HOPs2 of HOPs_12_49 library:
|
Reagent
|
Initial
|
Final
|
1 reaction (μl)
|
| Kapa Buffer A |
10x |
1x |
10 |
| Forward |
10 uM |
0.4 uM |
4 |
| Reverse (T7) |
10 uM |
0.4 uM |
4 |
| dNTP mix |
10 mM |
0.4 mM |
4 |
| Kapa Taq |
|
|
1 |
| Raw library |
2 ng/ul |
1µl per 10µl of reaction |
10 |
| Water (up to 100 ul) |
|
|
67 |