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Protocols

Older libraries PCR protocol

Jan 14, 2021, 12:02 PM by Guy Nir

C01beta NonHOPs library:

Reagent Initial Final 1 reaction (μl)
Kapa Buffer A 10x 1x 10
Forward 10 uM 0.4 uM 4
Reverse (T7) 10 uM 0.4 uM 4
dNTP mix 10 mM 0.4 mM 4
Kapa Taq 1
Raw library 0.2 ng/ul 1µl per 10µl of reaction 10
Water (up to 100 ul) 67

C01beta HOPs library and HOPs1 of HOPs_12_49 library:

Reagent Initial Final 1 reaction (μl)
Kapa Buffer A 10x 1x 10
Forward 10 uM 0.4 uM 8
Reverse (T7) 10 uM 0.4 uM 8
dNTP mix 10 mM 4
Kapa Taq 1
Raw library 2 ng/ul 1µl per 10µl of reaction 10
Water (up to 100 ul) 59

HOPs2 of HOPs_12_49 library:

Reagent Initial Final 1 reaction (μl)
Kapa Buffer A 10x 1x 10
Forward 10 uM 0.4 uM 4
Reverse (T7) 10 uM 0.4 uM 4
dNTP mix 10 mM 0.4 mM 4
Kapa Taq 1
Raw library 2 ng/ul 1µl per 10µl of reaction 10
Water (up to 100 ul) 67
Protocols Page: 1  | 2