Cell Line Authentication (STR Analysis)
To provide for compliance with the NIH policies on enhancing reproducibility through rigor and transparency (NOT-OD-15-103), the Molecular Genomics (MG) Core(MRB 6.160) will begin offering STR analysis of genomic DNA isolated from human cells maintained in culture. Short Tandem Repeat (STR) analysis is a well-established technique for assessing cell line identity and has been the subject of a comprehensive evaluation (ASN-0002) by the ATCC Standard Development Organization (SDO). STR analysis will use a PCR-based strategy to target nine human STR loci and the amelogenin gene (gender determination). Amplicons are analyzed using capillary electrophoresis on an ABI genomic analyzer and the resulting profile is compared to the STR database maintained by ATCC. The results from this analysis can be used in providing evidence of compliance for the cell line authentication that is being requested by the NIH and many scientific journals.
STR AnalysisForm (DOC)
Cost: $50.00/ cell sample
Please provide 2-3 up of genomic DNA in 10-20 ul sterile water.
Questions? Please contact Dr. Steve Widen @ firstname.lastname@example.org