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Nex-Gen Core: Next Generation Sequencing Core Facility

UTMB’s Next Generation Sequencing (NGS) Core provides high throughput sequencing to UTMB investigators using twodedicated Illumina instruments; NextSeq 550 and a MiniSeq. The NGS Core offers support in library construction from various template sources; RNA (total, poly A+ and miRNA), chromatin immunoprecipitated DNA (ChIP-Seq) and DNA (genomic and amplicon-derived). Library complexity is assessed using qPCR prior to amplification. Illumina NGS technology uses adapter-ligated template molecules to populate a hollow glass flow cell. Individual target molecules are then amplified to create template clusters. Templates are sequenced using reversible, fluorescent-tagged terminator nucleotides. Costs are reduced by indexing (“bar coding”) individual template libraries, allowing multiple libraries to be sequenced in each of the flow cell lanes. 

What can you do with Next Generation Sequencing?
  • ChIP-Seq
  • De novo assembly
  • Variant identification
  • SNPs
  • Transcriptome (reference-guided or de novo)
  • miRNA and small ncRNA sequencing
  • Quantitative - RNA-Seq expression analysis
  • Exome Sequencing (Cancer or Custom Panels)
Request Forms

ISC Instructions

Next Generation Sequencing Costs: SEPTEMBER 3, 2018
Run Cost    
PlatformRun TypeEstimated ReadsCost
NxSeq550   
 SE 75 Base130M$1,585.00
 SE 150 base130M$1,735.00
 PE 75 base130M$1,785.00
 PE 150 base130M$2,230.00
 SE 75 base400M$1,979.00
 SE 150 base400M$3,304.00
 PE 75 base400M$3,404.00
 PE 150 base400M$4,976.00
MiniSeq         
 75 cycles25M$1,187.00
 150 cycles25M$1,325.00
 300 cycles25M$1,891.00
 300 cycles8M$922.00

M, million; SE, single end; PE, paired end

Library Cost 
RNA-Seq Non-Stranded, no dT selection$276.00
RNA-Seq Non-Stranded & dT Selected$282.00
RNA-Seq Stranded & dT Selected$304.00
RNA-Seq RiboZero rRNA Depletion$373.00
ChIP-Seq$269.00
DNA-Seq (Covaris)$274.00
DNA-Seq  Nextera Flex$278.00
microRNA (Small RNA)$296.00

Staff:

Jill K. Thompson
Steven G. Widen, PhD

Location: 2.110 Basic Science Bldg.
Contact: sgwiden@utmb.edu
Phone: (409) 772-6349

Please contact Dr. Widen to discuss your sequencing project.