ngs

Nex-Gen Core: Next Generation Sequencing Core Facility

UTMB’s Next Generation Sequencing (NGS) Core provides high throughput sequencing to UTMB investigators using three dedicated Illumina instruments; HiSeq1500, NextSeq 550 and a MiniSeq. The NGS Core offers support in library construction from various template sources; RNA (total, poly A+ and miRNA), chromatin immunoprecipitated DNA (ChIP-Seq) and DNA (genomic and amplicon-derived). Library complexity is assessed using qPCR prior to amplification. Illumina NGS technology uses adapter-ligated template molecules to populate a hollow glass flow cell. Individual target molecules are then amplified to create template clusters. Templates are sequenced using reversible, fluorescent-tagged terminator nucleotides. Costs are reduced by indexing (“bar coding”) individual template libraries, allowing multiple libraries to be sequenced in each of the flow cell lanes. 

What can you do with Next Generation Sequencing?
  • ChIP-Seq
  • De novo assembly
  • Variant identification
  • SNPs
  • Transcriptome (reference-guided or de novo)
  • miRNA and small ncRNA sequencing
  • Quantitative - RNA-Seq expression analysis
  • Exome Sequencing (Cancer or Custom Panels)

Request Forms

ISC Instructions


Next Generation Sequencing Costs: October 30, 2017

Library Cost

RNA-Seq Non-Stranded, no dT selection$262.00
RNA-Seq Non-Stranded & dT Selected$265.00
RNA-Seq Stranded & dT Selected$277.00
rRNA Depletion  (additional cost)$88-98.00
ChIP-Seq$242.00
DNA-Seq (Covaris)$262.00
DNA-Seq  Nextera$298.00
microRNA (Small RNA)$270.00


PlatformRun TypeEstimated ReadsCost
Full 8PE 50 base150M$2,206.00
RapidSE 50 base240M$2,025.00
 SE 100 base240M$2,625.00
 SE 150 base240M$3,225.00
 PE 50 base240M$3,125.00
 PE 100 base240M$3,880.00
NxSeq550SE 50-150 base130M$1,324.00
 PE 75 base130M$1,324.00
 PE 150 base130M$1,965.00
 SE 75 base400M$2,110.00
 SE 150 base400M$3,240.00
 PE 75 base400M$3,440.00
 PE 150 base400M$5,110.00
MiniSeq75 cycles25M$1,089.00
 150 cycles25M$1,229.00
 300 cycles25M$1,809.00
 300 cycles8M$814.00
M, million; SE, single end; PE, paired end

Staff:

Jill K. Thompson
Stephen G. Widen, PhD
Thomas G. Wood, PhD

Location: 6.156 Medical Research Building
Contact: tgwood@utmb.edu
Phone: (409)747-0387

Please contact the Dr. Wood or Dr. Widen to discuss your sequencing project.