
Nex-Gen Core: Next Generation Sequencing Core Facility
UTMB’s Next Generation Sequencing (NGS) Core provides high throughput sequencing to UTMB investigators using twodedicated Illumina instruments; NextSeq 550 and a MiniSeq. The NGS Core offers support in library construction from various template sources; RNA (total, poly A+ and miRNA), chromatin immunoprecipitated DNA (ChIP-Seq) and DNA (genomic and amplicon-derived). Library complexity is assessed using qPCR prior to amplification. Illumina NGS technology uses adapter-ligated template molecules to populate a hollow glass flow cell. Individual target molecules are then amplified to create template clusters. Templates are sequenced using reversible, fluorescent-tagged terminator nucleotides. Costs are reduced by
indexing (“bar coding”) individual template libraries, allowing
multiple libraries to be sequenced in each of the flow cell lanes.
Request Forms
ISC Instructions
Next Generation Sequencing
Costs: January 1,2024
Run Cost | | | |
Platform | Run Type | Estimated Reads | Cost |
NextSeq 2000 | SE 100 Base | 100M | $1360.00 |
| PE 150 Base | 100M | $1690.00 |
| PE 300 Base | 100M | $2310.00 |
| SE 100 Base | 400M | $1900.00 |
| PE 100 Base | 400M | $3400.00 |
| PE 150 Base | 400M | $4000.00 |
| PE 300 Base | 300M | $4300.00 |
| SE 50 Base | 1200M | $2700.00 |
| SE 100 Base | 1200M | $3700.00 |
| PE 100 Base | 1200M | $4900.00 |
| PE 150 Base | 1200M | $6400.00 |
NxSeq550 | | | |
| SE 75 Base | 130M | $1,650.00 |
| SE 150 base | 130M | $1,750.00 |
| PE 75 base | 130M | $1,800.00 |
| PE 150 base | 130M | $2,665.00 |
| SE 75 base | 400M | $2,220.00 |
| SE 150 base | 400M | $3,895.00 |
| PE 75 base | 400M | $3,920.00 |
| PE 150 base | 400M | $6,165.00 |
MiniSeq | | | |
| 75 cycles | 25M | $1,320.00 |
| 150 cycles | 25M | $1,490.00 |
| 300 cycles | 25M | $2,210.00 |
| 300 cycles | 8M | $980.00 |
M, million; SE, single end; PE, paired end
NGS Data Analysis $85/hr
Library Cost | |
RNA-Seq Non-Stranded, no dT selection | $276.00 |
RNA-Seq Non-Stranded & dT Selected | $282.00 |
RNA-Seq Stranded & dT Selected | $304.00 |
RNA-Seq RiboZero rRNA Depletion | $373.00 |
ChIP-Seq | $269.00 |
DNA-Seq (Covaris) | $274.00 |
DNA-Seq Nextera Flex | $278.00 |
microRNA (Small RNA) | $296.00 |
Staff:
Jill K. Thompson
Haiping Hao, PhD
Location: 2.110 Basic Science Bldg.
Contact: hahao@utmb.edu
Phone: (409) 772-6349
Please contact Dr. Hao to discuss your sequencing project.