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 Ordering Blood Components and Laboratory Testing

RBCimage

NOTICE:

 

Blood and blood components must be ordered by a Licensed Independent Practitioner (LIP) such as a physician or nurse practitioner. Transfusions should only be prescribed for a patient when the benefit of transfusion is likely to outweigh the risk.

See link below for Transfusion Services STAT Turn Around Times. 

Transfusion Services STAT Turn Around Times.docx

  • RBC Transfusion Guidelines
       Indications For Transfusing RBC's 
    Adult and Pediatric 
    Hemoglobin (Hgb) Concentration (g/dL)Patient Population/ Clinical
    < 7.0Non-Bleeding Medical and Surgical patients, including critically ill but clinically stable ICU patients
    < 7.5Patients undergoing cardiac surgery
    < 8.0

    Patients with Acute Coronary Syndrome, Patients with hip fracture and cardiovascular disease and Hemodynamically stable patients with acute GI bleeding

  • Plasma Transfusion Guidelines
    Indications For Transfusing Plasma 
    With at least 1 of the following: Patient Population/ Clinical
    PT > 20 sActive Bleeding
    aPTT > 57 sSurgery or Invasive Procedure
    Specific Clotting factor deficiency < 26% of normal and no other product available

    Dilutional Coagulopathy (i.e. Massive Transfusion)


  • Platelet Transfusion Guidelines
    Indications for Transfusing Platelets: Laboratory and Clinical 
    Platelet Concentration (K/μL)Patient Population/ Clinical
    < 10
    • Clinically stable non-bleeding inpatients
    < 20
    • Inpatients

      • On anticoagulation prophylaxis (e.g. heparin, DOAC, etc.)

      • Immediately prior to low risk invasive procedures, including but not limited to:

        • Thoracentesis

        • Temporary non-tunneled venous access insertion and removal including PICC lines

        • Peripheral arterial line

        • Bone marrow biopsy

      • About to be discharged

    • Outpatients

    < 50
    • Active bleed

    • Lumbar Puncture

    • Immediately prior or during high-risk procedures such as those listed in JVIR guidance.


    < 100
    • Neurosurgical patients

    • CNS or retinal bleed

    • Bleeding due to platelet dysfunction not responsive to DDAVP or Cryo (must consult Transfusion Medicine Physician)

    Any
    • Platelet dysfunction (documented aspirin)

    • Massive hemorrhage

     

  • Component TAT
    PRODUCT PROCEDUREPRE-REQUISITETAT

    Type and Screen

    Receipt of acceptable specimen; Negative Antibody Screen and No History of Antibody*

    60 minutes

    ABORh Confirmation

    Receipt of acceptable specimen

    30 minutes

    Crossmatch

    (Red Blood Cells)

    Receipt of acceptable specimen; Complete Type and Screen with Negative Antibody Screen and No History of Antibody ** Complete 2nd ABO/Rh Confirmation

     

    15 minutes

    Crossmatch additional units

    Receipt of acceptable specimen; Complete Type and Screen with Negative Antibody Screen and No History of Antibody ** Complete 2nd ABO/Rh Confirmation

     

    15 minutes

    Uncrossmatched RBCs –

    O Negative

    Verbal confirmation that physician will sign waiver; collection of specimen

    10 minutes

    Uncrossmatched RBCs –

    Type Specific

    Verbal confirmation that physician will sign waiver; Receipt of specimen and 2nd ABO/Rh Confirmation

    10 minutes

     

    Fresh Frozen Plasma

    Blood type on record for current admission

    30 minutes

    If no blood type on record on current admission and receipt of acceptable specimen.

    1 hour

    Platelets

    Blood type on record for current admission

    15 minutes

     

    If no blood type on record on current admission and receipt of acceptable specimen.

    1 hour

    Cryoprecipitate (pooled)

    Blood type on record for current admission

    15 minutes

    If no blood type on record on current admission and receipt of acceptable specimen.

    1 hour

    *All TATs dependent on availability of blood component.

    Special transfusion requirements (ex.: CMV negative, Irradiated, Hgb S negative, will lengthen TAT).

    **If antibody screen in positive, TAT dependent on complexity of antibody ID.

Pre-Transfusion Testing

Correctly identifying the patient during collection of the pre-transfusion sample is vital in avoiding 'wrong blood to wrong patient' episodes.

All specimen collection and labeling must be done in accordance with the UTMB IHOP Policy 09.13.29 - Transfusion of Blood Components.

Pre-Transfusion compatibility testing prior to transfusion involves the following:

TYPE & SCREEN TESTING

  • ABO/RH Identification
  • Red Cell Antibody Screen to detect red cell antibodies
    • For patients who have 'no red cell antibodies detected,' compatible units are selected and issued electronically.
    • For patients who have 'red cell antibodies detected,' further laboratory work is required to identify the specificity of the antibody, to type the patient and donor units in order to provide specific antigen negative blood and to perform a full serological crossmatch. For patients with multiple antibodies, this work can take several hours to complete. 
    Two ABO/RH identifications MUST be on file to obtain blood components. Otherwise, emergency units and an emergency release waiver will be needed. 

ABORH CONFIRMATION TESTING

  • ABO/RH Confirmation

 

NOTE: Having a T&S ready and available for patients who may need a transfusion allows for quicker dispensing of necessary blood component when the need is determined.

Specimen Expiration:

Compatibility testing (Type and Screen sample collected to be used for transfusion) is to be repeated every 3 days, as indicated. The Type and Screen sample expires at midnight on the 3rd day after it was drawn. 

  • For Adults: The specimen expires at midnight 3 days after the specimen was collected. (Example, collected Tuesday, would expire Friday at 23:59).
  • For Neonates ≥ 4 months of age, the expiration of the compatibility specimen follows the same principle as adults.
  • For infants < 4 months of age, the specimen expires when the infant turns 4-months of age or is discharged, whichever comes first.

Note: If a patient is discharged or transferred from another UTMB facility, they will require a new Type and Screen collection.

Reminder: If the antibody screen is positive there will be an inherent delay to find the correct component that is compatible for the patient. The delay can range from 1-2 hours or possibly even days depending on the complexity.

 

Specimen Requirements:

  • All requests must include requesting physician's name and ID number, patient's complete name, UH number, current account number, patient location, clinical information/diagnosis, identity of phlebotomist and verifier, and date/time of sample collection.

  • The specimen must be labeled with the patient's full name and UH number. Specimens should be transported to the laboratory in a biohazard specimen bag with the request form in the pocket of the bag. All information must be complete and legible on the specimen and the requisition.

Causes for Specimen Rejection

  • No label on tube
  • Incorrect or incomplete patient identifiers
  • Incorrect or incomplete collection information
  • Wrong tube type
  • Gross hemolysis
  • Diluted specimen
  • QNS

NOTE- If a specimen is rejected; a Blood Bank technologist will contact the nurse or physician of the rejection and need for re-collection.

Expiration of The Specimen

The expiration of a compatibility specimen only applies to Packed Red Blood Cells (PRBCS).

  • For adults: the specimen expires 3 days after the specimen was collected. For example, if the sample was collected on Tuesday, it would expire Friday at 23:59.
  • For infants ≥ 4 months of age, the expiration of the compatibility specimen follows the same principle as adults.
  • For infants < 4 months expires when the infant turns 4 months of age.

Useful Resources

Transfusion Services Quick Tools