Biorepository and Mass Spectrometry Laboratory
The Biorepository and Mass Spectrometry Laboratory (~3,000 sq ft space) is located on the 10th floor of the Medical Research Building. It has a satellite laboratory (approximately 300 sq ft) for the initial processing of human samples located on the 4th floor of the John Sealy Annex Building. The inter-connecting labs in the MRB allow for science collaboration among researchers interested in muscle biology and metabolism in aging.
Analytical Services
- Sample biorepository and tracking
- Measurement of stable isotope enrichment in biological samples by mass spectrometry (protein, lipid and glucose metabolism)
- Metabolic modeling
Equipment
- one Agilent Technologies 6890 Gas Chromatograph 5973N Mass Spectrometers with EI and autosampler
- one Agilent Technologies 6890N Gas Chromatograph 5973N Mass Spectrometers with EI/PCI/NCI bundle and autosampler
- one Agilent Technologies 6890N Gas Chromatograph 5975B Mass Spectrometers with EI and autosampler
- two Agilent Technologies 6890A Gas Chromatograph 5975C Mass Spectrometers with EI and autosampler
- one Agilent Technologies 6890 Gas Chromatograph equipped with Flame Ionization Detector and autosampler
- one Thermo Delta V Gas Chromatograph-Combustion-Isotope Ratio Mass Spectrometer with GasBench for measurement of very low enrichments of deuterium, 13C, 15N, 18O, in muscle, blood and breath
- three Speed-vacuum systems for sample concentration and desiccation
- one nitrogen evaporator (N-Evap) for sample desiccation
- one Sorvall microcentrifuge
- one Jouan bench centrifuge to separate blood subfractions
- one Spectrophotometer
- one MiniProtean electrophoresis system with blotting apparatus
- nine -80° C freezers
- one -20° C freezer
- two 4 C° refrigerator for sample and reagent storage
- one 121 L liquid nitrogen dewar for tissue sample storage
- one Biosafety sterile cabinet
- four fume hoods
- standard lab items (e.g. water-baths, microplate reader, rotators, shakers, sonicators, high-speed and micro centrifuges)