Brendan Prideaux , PhD Assistant Professor

prideaux--brendan11Department of Neurobiology 
Research Bldg. 17, 5.212F 
Route: 0620 | Tel: (409) 772-1092 |

Education and Training

2003 – 2007 - PhD ‘Applications of Imaging MALDI-MS to the Determination of Hazardous Compounds in Skin’ Sheffield Hallam University, UK 
1999 – 2003 - BSc Biomedical Chemistry (Hons), Sheffield Hallam University, UK

Research Interests

My research is focused on understanding pathogenic mechanisms of infectious disease using state-of-the-art mass spectrometry and imaging technologies.

Elucidating host-pathogen interactions at the cellular level is of vital importance for discovery and development of drugs for both host-directed and/or pathogen-based therapeutic approaches. Mass Spectrometry Imaging (MSI) is a label free technique that utilizes the sensitivity and selectivity of mass spectrometry to visualize the distribution of molecules in biological tissues at cellular detail. Using this approach analytes such as lipids (lipidomics), metabolites (metabolomics), and drugs can be identified and localized within target tissues and cells.

We are applying MSI-based lipidomic and metabolomic techniques to study the in-situ pathogenesis of many viral and bacterial infectious diseases including HIV, Tuberculosis, Cerebral Malaria, Meliodosis, and Pneumonic Plague.

Schematic of Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging (MALDI-MSI)

Schematic of Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging (MALDI-MSI)

Selected Publications

Prideaux B., Lenaerts A., Dartois V. Imaging and spatially resolved quantification of drug distribution in tissues by mass spectrometry. Curr Opin Chem Biol. (2018) 44, 93-100

Blanc L., Lenaerts A., Dartois V., Prideaux B. Visualization of Mycobacterial Biomarkers and Tuberculosis Drugs in Infected Tissue by MALDI-MS Imaging. Anal Chem. (2018) 90, 6275-6282

Zimmerman M., Blanc L., Chen P.Y., Dartois V., Prideaux B. Spatial Quantification of Drugs in Pulmonary Tuberculosis Lesions by Laser Capture Microdissection Liquid Chromatography Mass Spectrometry (LCM-LC/MS). J Vis Exp. (2018) 134

Rifat D., Prideaux B., Savic R.M., Urbanowski M.E., Parsons T.L., Luna B., Marzinke M.A., Ordonez A.A., DeMarco V.P., Jain S.K., Dartois V., Bishai W.R., Dooley K.E. Pharmacokinetics of rifapentine and rifampin in a rabbit model of tuberculosis and correlation with clinical trial data. Sci Transl Med. (2018) 10, eaai7786

Zhao Y., Prideaux B*., Nagasaki Y., Lee M.H., Chen P.Y., Blanc L., Ho H., Clancy C.J., Nguyen M.H., Dartois V., Perlin D.S. Unraveling Drug Penetration of Echinocandin Antifungals at the Site of Infection in an Intra-Abdominal Abscess Model. Antimicrob Agents Chemother. (2017) 61, pii: e01009-17 *Equal contribution

Marakalala M.J., Raju R.M., Sharma K., Zhang Y.J, Eugenin E.A., Prideaux B., Daudelin I.B., Chen P.Y., Booty M.G., Kim J.H., Eum S.Y., Via L.E., Behar S.M., Barry C.E. 3rd, Mann M., Dartois V., Rubin E.J. Inflammatory signaling in human tuberculosis granulomas is spatially organized. Nat Med. (2016) 22, 531-8.

Prideaux B., Via L.E., Zimmerman M.D., Eum S., Sarathy J., O'Brien P., Chen C., Kaya F., Weiner DM., Chen P.Y., Song T., Lee M., Shim T.S., Cho J.S., Kim W., Cho S.N., Olivier K.N., Barry C.E. 3rd, Dartois V. The association between sterilizing activity and drug distribution into tuberculosis lesions, Nat Med. (2015) 10, 1223-7.

Prideaux B., ElNaggar M.S., Zimmerman M., Wiseman J.M., Li X., Dartois V. Mass spectrometry imaging of levofloxacin distribution in TB-infected pulmonary lesions by MALDI-MSI and continuous liquid microjunction surface sampling. Int. J. Mass Spectrom. (2015) 377, 699–708

Prideaux B., Stoeckli M. Mass Spectrometry Imaging for Drug Distribution Studies, J. Proteomics. (2012) 75, 4999-5013.

Prideaux B., Dartois V., Staab D., Weiner D., Goh A., Via L.E., Barry C.E., Stoeckli M. High-Sensitivity MALDI-MRM-MS Imaging of Moxifloxacin Distribution in Tuberculosis-Infected Rabbit Lungs and Granulomas, Anal. Chem. (2011) 83, 2112-2118.

Solon E., Schweitzer A., Stoeckli M., Prideaux B., Autoradiography, MALDI-MS, and SIMS-MS Imaging in Pharmaceutical Discovery and Development, AAPS J. (2010) 12, 11-26

Link to Pubmed Publications