Research Interests
Dr. Emmett's current research specializes in identification of
novel therapeutic targets and biomarkers in oncology (esp.
neuro-oncology). Dr. Emmett utilizes high-resolution mass
spectrometry (FT-ICR MS) and develops novel chromatographic and MS
based methodology for high sensitivity endogenous biomolecule analysis. Dr. Emmett
has established a multi-disciplinary research group enabling a systems
biological approach integrating geneomics, transcriptomics, proteomics,
glycomics, lipidomics, metabolomics, phenotypic responses with computational mathematical data analysis to the study of cancer focusing on the identification of novel biomarkers and therapeutic targets.
Recent Publications
- Proteomic Investigation of Glioblastoma Cell Lines Treated
with Wild-Type p53 and Cytotoxic Chemotherapy Demonstrates an
Association between Galectin-1 and p53 Expression, Maja Puchades, Carol
L. Nilsson, Mark R. Emmett, Kenneth D. Aldape, Yongjie Ji, Frederick
F. Land, Ta-Jen Liu, and Charles A. Conrad, Journal of Proteome
Research, 2007, 6: 869-875.
- Method for Lipidomic Analysis: p53 Expression Modulation of
Sulfatide, Ganglioside and Phospholipid Composition of U87 MG
Glioblastoma Cells, Huan He, Charles A. Conrad, Carol L. Nilsson,
Yongjie Ji, Tanner M. Schaub, Alan G. Marshall and Mark R. Emmett,
Analytical Chemistry, 2007, 79: 8423-8430.
- KIT Kinase Mutants Show Unique Mechanisms of Drug Resistance
to Imatinib and Sunitinib in Gastrointestinal Stromal Tumor Patients,
Gajiwala, K. S.; Wu, J. C.; Christensen, J.; Deshmukh, G. D.; Diehl,
W.; DiNitto, J. P.; English, J. M.; Greig, M.; He, Y.-A.; Jacques, S.
L.; Lunney, E. A.; McTigue, M; Molina, D.; Quenzer, T. A.; Wells, P.
A.; Yu, X.; Zhang, Y.; Zou, A.; Emmett, M. R.; Marshall, A. G.; Zhang,
H.-M.; Demetri, G. Proc. Natl. Acad. Sci. U.S.A. 2009, 106,
1542-1547.
- Polar lipid remodeling and increased sulfatide expression
are associated with the glioma therapeutic candidates, wild type p53
elevation and the topoisomerase-1 inhibitor, Irinotecan, Huan He, Carol
L. Nilsson, Mark R. Emmett, Yongjie Ji, Alan G. Marshall, Roger A.
Kroes, Joseph R. Moskal, Howard Colman, Frederick F. Lang, and Charles
A. Conrad, Glycoconjugates. 2010, 27(1): 27-38.
- Glycomic and transcriptomic response of GSC-11 glioblastoma
stem cells to STAT-3 phosphorylation inhibition and serum-induced
differentiation, Huan He, Carol L. Nilsson, Mark R. Emmett, Alan G.
Marshall, Roger A. Kroes, Joseph R. Moskal, Yongjie Ji, Howard Colman,
Waldemar Priebe, Frederick F. Lang, and Charles A. Conrad, J.
Proteomics Research, 2010, 9: 2098-2108.
- Overexpression of ST6GalNAcV, a ganglioside-specific a2,6
sialyltransferase, inhibits glioma invasivity. Roger A. Kroes, Huan
He, Mark R. Emmett, Carol L. Nilsson, Alan G. Marshall, and Joseph R.
Moskal, Proceedings of the National Academy of Sciences, 2010, 107
(28): 12646-12651.
- Liquid Chromatography Electrospray Ionization Fourier
Transform Ion Cyclotron Resonance Mass Spectrometric Characterization of
N-Linked Glycans and Glycopeptides, Xu Wang, Mark R. Emmett, and Alan
G. Marshall, Analytical Chemistry, 2010, 82, 6542-6548.
- New Reagents for Enhanced Liquid Chromatographic Separation
and Charging of Intact Protein Ions for Electrospray Ionization Mass
Spectrometry, Santosh G. Valeja, Jeremiah D. Tipton, Mark R. Emmett and
Alan G. Marshall, Analytical Chemistry, 2010, 82, 7515-7519.
- Computing H/D-Exchange rates of single residues from data of
proteolytic fragments, Ernst Althaus, Stefan Canzar, Carsten Ehrler,
Mark R. Emmett, Andres Karrenbauer, Alan G. Marshall, Anke Meyer-Baese,
Jeremiah D. Tipton and Huimin Zhang, BMC Bioinformatics, 2010, 11: 424-436.
- Determining and interpreting correlations in lipidomic
networks found in glioblastoma cells, Robert Gorke, Anke Meyer-Base,
Dorothea Wagner, Huan He, Mark R. Emmett, Charles A. Conrad, BMC Systems Biology, 2010, 4: 126-139. http:www.biomedcentral.com/1752-0509/4/126.
- Complexation and Calcium-Induced Conformational Changes in
the Cardiac Troponin Complex Monitored by Hydrogen/Deuterium Exchange
and FT-ICR Mass Spectrometry, George M. Bou-Assaf, Jean E. Chamoun,
Mark R. Emmett, Piotr G. Fajer, and Alan G. Marshall, 2010, International Journal of Mass Spectrometry, 2010, 302: 116-124.
- High Mass Accuracy and Resolution Facilitate Identification
of Glycosphingolipids and Phospholipids, Huan He, Mark R. Emmett, Carol
L. Nilsson, Charles A. Conrad and Alan G. Marshall, 2011, International Journal of Mass Spectrometry, 305: 116-119.
- Characterization of the Phosphoproteome in Androgen-Repressed
Human Prostate Cancer Cells by Fourier Transform Ion Cyclotron
Resonance Mass Spectrometry, Xu Wang, Paul A. Stewart, Qiang Cao,
Qing-Xiang Amy Sang, Leland W.K. Chung, Mark R. Emmett and Alan G.
Marshall, Journal of Proteome Research, 2011, 10: 3920-3928.
- Epitope Mapping of a 95 kDa Antigen in Complex with Antibody
by Solution-Phase Amide Backbone Hydrogen/Deuterium Exchange Monitored
by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry, Analytical Chemistry, 2011, 83: 7129-7136.
- Novel Polar Aprotic Modifiers Enhance Chromatographic
Separation, Increase Throughput, and Reduce Back-Exchange for Protein
Hydrogen/Deuterium Exchange Monitored by Fourier Transform Ion Cyclotron
Resonance Mass Spectrometry, Santosh G. Valeja1, Mark R. Emmett1,2,*,
and Alan G. Marshall, 2011, Journal of American Society for Mass Spectrometry, 2012, 23: 6634-6637.637
- Protein Expression in Glioma-Derived Stem-Like Cells
correlated to Gene Expression Data and Patient Outcomes, Nilsson, Carol
L;Sulman, Erik; Liu, Huiling; Wild, Christopher; Lichti, Cheryl F.;
Emmett, Mark R.; Lang, Frederick F.; Conrad, Charles A., Neuro-Oncology, 2012, 14: 96-97
- D-Glucose and D-mannose-based metabolic probes. Part 3.
Synthesis of specifically deuterated D-glucose, D-mannose and
2-deoxy-D-glucose. Izabela Fokt, Stanislaw Skora, Charles Conrad,
Timothy Madden, Mark Emmett, and Waldemar Priebe, Carbohydrate Research, 2013, 368: 111-119
- Integrative Biological Analysis For Neuropsychopharmacology,
Mark R. Emmett, Roger A. Kroes, Joseph R. Moskal, Charles A. Conrad,
Waldemar Priebe, Fernanda Laezza, Anke Meyer-Baese and Carol L.
Nilsson, Neuropsychopharmacology REVIEWS, 2014, 39: 5-23.