Research Interests
My research interests mainly focus on signaling mechanism of
innate immune response during airway inflammation using molecular
biology, cell biology, system biology, biochemical, and dynamic imaging
approaches. One of my major concentrations is to understand signaling
pathways controlling the "master" regulator of inflammation known as
nuclear factor-kB (NF-kB) signaling networks in airway epithelial
cells in response to viral infection, cytokine stimulation, and double
stranded RNA exposure. Most recently, we established a model of stable
type II epithelial-mesenchymal transition (EMT) induced by chronic
TGFb stimulation of telomerase-immortalized primary human small airway
epithelial cells (hSAECs) and we are using this model to actively
explore the pathogenesis and therapeutic interventions of severe asthma
associated with fibrosis.
Peer Reviewed Manuscripts
- Tian B, Zhang YH, Luxon BA, Garofalo RP,
Casola A, Sinha M, Brasier AR, Identification of NF-kappa B-dependent
gene networks in respiratory syncytial virus-infected cells, J Virol 76
(13): 6800-6814 July 2002
- Tian B, Nowak D., and Brasier AR. A TNF
Induced Gene Expression Program Under Oscillatory NF-kB Control. BMC
Genomics, 2005; 6:137. PMCID: PMC1262712.
- Tian B, Nowak D, Jamaluddin M, Wang S, and
Brasier AR. Identification Of Direct Genomic Targets Downstream Of The
NF-kB Transcription Factor Mediating TNF Signaling. J.Biol. Chem.,
2005; 280:17435-17448. PMID: 15722553.
- Nowak DE, Tian B, Jamaluddin M, Boldogh I,
Vergara LA, Choudhary S, and Brasier AR. Rel A Ser276 Phosphorylation
is required for activation of a subset of NF-kB Dependent Genes by
Recruiting Cdk-9/Cyclin T1 Complexes. Mol Cell Biol, 28: 3623-3638,
2008. PMC2423290
- Jamaluddin M, Tian B, Boldogh I, Garofalo RP
and Brasier AR Respiratory Syncyctial Virus Infection Induces A
ROS-MSK1-Phospho-Ser-276 RelA Pathway Required For Cytokine Expression.
J Virol, 83: 10605-10615, 2009. PMCID: PMC2753134.
- Choudhary S., Fang L, Tian B, Wu Z,
Rosenblatt KP, and Brasier AR. High Throughput siRNA Screening of the
Human Kinome Identifies Novel Kinases Controlling the Canonical NF-kB
Activation Pathway. J. Biol. Chem, 286: 37187-95, 2011. PMCID:
3199466.
- Brasier AR, Tian B, Jamaluddin M, Kalita MK,
Garofalo RP, and Lu M. RelA Ser 276 Phosphorylation-Coupled Lys 310
Acetylation Controls Transcriptional Elongation Of Inflammatory
Cytokines In RSV Infection. J Virol, 85: 11752-11769, 2011.
PMCID:PMC3209292.
- Kalita M, Sargysan K, Tian B, Paulucci A,
Najm H, DeBusschere B, and Brasier AR. Sources of Cell-to-cell
Variability in Canonical Nuclear Factor-kB (NF-kB) Signaling Pathway
Inferred from Single Cell Dynamic Images. J. Biol. Chem, 286:37741-57,
2011. PMCID: PMC3199517.
- Tian B, Zhao Y., Kalita M., Edeh M,
Paessler S, Casola A. Teng MF, Garofalo RP and Brasier AR.
CDK9-dependent transcriptional elongation in the innate ISG response to
RSV infection in airway epithelial cells. J Virol. 2013 Jun;
87(12):7075-92.
- Kalita M, Tian B, Gao B, Choudhary S, Wood
TG, Carmical JR, Boldogh I, Mitra S, Minna JD, Brasier AR. Systems
approaches to modeling chronic mucosal inflammation. Biomed Res Int.
2013
- Bertolusso R, Tian B, Zhao Y, Vergara L,
Sabree A, Iwanaszko M, Lipniacki T, Brasier AR, and Kimmel M. Dynamic
cross talk model of the epithelial innate immune response to
double-stranded RNA stimulation: coordinated dynamics emerging from
cell-level noise. PLoS One. 2014 Apr 7; 9(4): e93396
- Li X, Zhao Y, Tian B, Jamaluddin M, Mitra
A, Yang J, Rowicka M, Brasier AR, Kudlicki A. Probabilistic Integrative
Approach to Inferring the Modulation of NF-kB/RelA Dependent
Transcription J Biol Chem. 2014 Apr 25;289(17):11927-44
Reviews
- Tian B and Brasier AR. Identification of an NF-kB dependent gene network. Recent Prog Horm Res, Edited by A. Means. 58:95-130, 2003
- Nowak DE. Tian B, Brasier AR. Novel
Two-Step Cross-linking method for Identification of NF-kB Gene Network
by Chromatin Immunoprecipitation. Biotechniques, 39: 715-725, 2005.
PMID: 16315372.
- Tian B and Brasier AR (2006). The Nuclear
Factor-kB (NF-kB) Gene Regulatory Network. Microarrays and
Transcription Networks, Edited by Frances Shannon and Sudha Rhao. ©
2005, Landes Bioscience, Eurekah.com.
- Brasier AR, Liu P, Tian B. and Choudhary. Signaling pathways in the host cell response to RSV infection. In Host Gene Responses to RNA Viral Infection, Edited by Decheng Yang, World Scientific Publishing, 2008.
- Tian B, Yang J, and Brasier AR. Two-step
Crosslinking for Analysis of Protein-Chromatin Interactions. Methods
in Molecular Biology, Transcriptional Regulation, Methods and
Protocols. A. Vercura, Editor. 2012; 809:105-20