IBC Inactivation Subcommittee

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Principal Investigators are required to obtain approval for inactivation procedures for CDC/USDA select agents and any biological material handled at BSL3 or BSL4 from the IBC Inactivation Review Subcommittee for future use prior to removal of material from the respective laboratory/facility. The submission must include the following information in order to be reviewed:

• Inactivation SOP
• Validation SOP
• In-house generated data supporting inactivation validation

To submit inactivation procedures, please use the following link. Please limit one inactivation method per submission.

• Inactivation Procedures Submission
  

Policies and Procedures

• Institutional Biosafety Committee Inactivation Review Subcommittee Policy
• Management of Agent Inactivation Standard Operating Procedure (SOP) Submissions

Example Inactivation SOPs

• Formalin Fixation of Animal Tissue
• Formalin Fixation of Single Cell Suspensions for FACS Analysis
• Formalin Fixation of Tissue Culture Plates or Slides
• Virus Inactivation of SDS-Heat and Protein Lysate Prep
• Virus Inactivation of Liquid Samples Trizol_TriPure
• Virus Inactivation of Tissues using the TissueLyser
• Virus Inactivation using RLT Buffer

Example Validation SOPs

• Validation of whole or partial organ fixation

Supplemental Information

• PI Inactivation Submission Guidance
• SA GRAM 12-21-2022: Validation of Inactivation Procedures for Regulated Nucleic Acids
• CDC Guidance on the Inactivation or Removal of Select Agents and Toxins for Future Use
• Biosafety in Microbiological and Biomedical Laboratories, current edition, Appendix K