Research

ALERT BAR

Bone Biology Laboratory

Faculty and Personnel

  • Zbigniew Gugala, MD, PhD - Associate Professor, Lab Director
  • Elizabetn Salisbury, PhD - Assistant Professor
  • Randal P. Morris, BS - Lab Manager

Overview and Facilities

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Research in the Orthopaedic Bone Biology Laboratory is focused on the endocrinological and molecular factors which influence the pace of bone turnover and how those factors can be modulated by environmental cues.

Work in the laboratory has also involved the physiology of fracture/wound healing, alkaline earth metabolism in laboratory animals and biomaterials such as charged resins. We seek to understand the interrelationships between bone and marrow cells which appear to play such an important role in the development of osteoporosis and other metabolic bone diseases.

adj_Research-MicroscopeThe lab recently acquired a new microscope, the Invitrogen™ EVOS™ FL Auto Imaging System, capable of live cell imaging, area scanning, image stitching, and time-lapse imaging. This imaging platform is also equipped with an environmental chamber that enables precise control of temperature, humidity, and three gases live cell imaging.

The lab is equipped with a SkyScan 1174 Micro CT scanner for three-dimensional microscopy. The scanner is capable of 6-15 micron spatial resolution in a 30mm x 80mm scanning area with software packages to generate CT reconstructions, 3D volumes, and volume analysis, and mineral density phantoms to accurately measure bone mineral density. This system is capable of a wide array of bone morphometry analysis including X-ray radiography, tomographic reconstruction, 3D-reconstruction, and 2D/3D quantitative analysis of bone densities.

adj_Research-MicroCTSeveral high-performance PC workstations are loaded with imaging software packages to allow image 3D visualization, reconstruction and analysis from CT, MRI, micro-CT, SPECT, or 3D ultrasound, as well as generating STL files for 3D reconstructions and subsequent 3D printing.

adj_Research-PrinterThe Bone Biology Lab is equipped with a Stratasys Objet Eden260VS 3D printer for ultra‐thin‐layer, 16 micron, high‐resolution, 3D printing. The Eden260VS Prime Design Series printer is based on a proprietary PolyJet Technology, and it offers 18 unique printing polymers with diverse and versatile material properties: Vero family – grey, black+, white+, blue, clear, DurusWhite; polypropylene‐like material, RGD720, Endur, Tango family – grey and black, and High Temperature. The 3D printer also offers printing in MED610, which can be used in biologic applications as a biocompatible part making.

adj_Research-BoseThe lab is also equipped with a Bose ElectroForce 3200 bi-axial mechanical testing system. This is a bi-axial system with up to 300 Hz frequency response ideally suited for biomechanical testing of mouse and rat specimens.

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