A. For the Fortessa: Your cells should be at a concentration of 20 million cells per mL. The smallest volume that is recommended for the Fortessa is 200uL.
For the Aria: Your cells can be at a concentration of 40 million cells per mL. The smallest volume that is recommended is 200uL. We always recommend that you bring extra FACS buffer so we can dilute the sample if we need to. It
is easier for us to dilute than concentrate the sample as we currently do not have a centrifuge.