The following resources are provided to support investigators at all experience levels. These links include introductory material, panel design tools, spectral viewers, software training, and specialized applications relevant to flow cytometry research.
Flow Cytometry Basics
Introductory resources for users new to flow cytometry or seeking a refresher on fundamental concepts:
- Introduction to Flow Cytometry – Basics Guide (Bio‑Rad)
- Fluorescence Tutorials (Thermo Fisher Scientific)
Panel Design Resources
Tools and references to assist with multicolor panel development and optimization:
- Optimized Multicolor Immunofluorescence Panels (Database from Cytometry Part A)
- FluoroFinder – Search antibodies from multiple vendors in one place
- Antigen Density for Human and Murine Surface Markers (Bio‑Rad)
Spectral Viewers
Online tools for visualizing fluorophore excitation and emission spectra to aid in panel design:
- Spectra Viewer (FPbase, great for Fluoroscent Proteins)
- Full Spectrum Viewer (Cytek)
- Spectrum Viewer (BD Biosciences)
- Spectrum Viewer (Thermo Fisher Scientific)
- Fluorescence Spectra Analyzer (BioLegend)
Data Analysis Software & Training
Resources supporting flow cytometry data analysis and high‑dimensional approaches:
- FlowJo Webinars (FlowJo)
- FlowJo Exchange – Free FlowJo plugins
- Comparison of Clustering Methods for High‑Dimensional Single‑Cell Flow and Mass Cytometry Data
- FlowSOM: Using Self‑Organizing Maps for Visualization and Interpretation of Cytometry Data
Specialized Applications
Resources highlighting advanced and specialized cytometry techniques:
- Apoptosis and Beyond: Cytometry in Studies of Programmed Cell Death
- RNA Flow Cytometry Using the Branched DNA Technique
Additional Training & Support
Investigators seeking hands‑on instruction, instrument training, or consultation support are encouraged to explore additional Core Facility training and consultation services or contact staff for guidance on selecting appropriate resources.