The following guides and protocols are provided to assist investigators with instrument operation, experimental setup, and standardized sample preparation. These resources are intended to support both new and experienced users and promote consistent, high‑quality data acquisition across experiments.
Instrument Guides
Step‑by‑step reference documents for commonly used instruments within the Core Facility:
- Fortessa / FACSymphony Conventional Flow Cytometry Guide
- FACSymphony Full Spectrum Flow Cytometry Guide
- High‑Throughput Sampler (HTS) Guide
- Bio‑Plex 200 System Guide
- Bio‑Plex Wash Station Guide
General Flow Cytometry Protocols
Standard protocols commonly used for flow cytometry experiments:
- Basic Staining Protocol
- Fc Block Protocol
- Basic Fixation Protocol
- Antibody Titration Protocol
- Live/Dead Fixable Stain Protocol
- Nucleic Acid Staining Protocol
(used for staining dead cells in live samples) - Live Cell Staining Protocol
(used for staining live cells intended for downstream applications)
Controls and Compensation Protocols
Protocols to support appropriate experimental controls and accurate data analysis:
- Unstained Control Protocol
- Single‑Color Control Protocol
- Fluorescence Minus One (FMO) Control Protocol
Additional Support
If you are unsure which guide or protocol is most appropriate for your experiment, or if you require protocol customization, investigators are encouraged to contact Core Facility staff or schedule a consultation for personalized assistance.