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UTMB Proteomics Center
Serving as the home for one of seven newly awarded national Proteomics Centers funded by the NHLBI, the Biomolecular Resource Facility at UTMB brings the power of multiple analytical technologies to proteomics research. The UTMB NHLBI Center for Airway Inflammation
consists of a multi-disciplinary team of scientists and
physician-scientists to study protein expression associated with
signaling pathways important in airway inflammation. In addition, the
Center is engaged in the development of innovative technologies for
application to proteomics research. It is the result of an NHLBI
initiative to establish centers to enhance and develop innovative
proteomics technologies and apply them to biological questions relevant
to heart, lung, blood, and sleep health and disease.
The Biomolecular Resource Facility (BRF) is a UTMB designated core
facility providing research support targeted to the analysis of
biomolecules, especially proteins and peptides. The initial organization
of the BRF began in 1975 and the facility has grown considerably over
the years to meet the analytical needs of biomedical investigators. Dr.
Alexander Kurosky, has been the Director of this facility since its
The overarching philosophy of the BRF is to provide UTMB
researchers with a menu of services encompassing state-of-the-art
analytical capabilities and technologies at reasonable costs that will
enhance their funded research projects as well as the quality of their
publications. Investigators are encouraged to interact closely with BRF
personnel to obtain maximum benefit of the BRF services. Technologies
and the available instrumentation in the BRF are at the cutting edge of
research. The BRF is committed to provide expert analyses, and Core
personnel have been well-trained and are highly skilled in their
analytical duties. In order to ensure quality of services, the BRF Cores
participate in the ABRF's annual analytical testing evaluations using
identical samples distributed to all member facilities.
Biomolecular Resource Facility
1.106 Libbie Moody Thompson Basic Science Building
301 University Boulevard
Galveston, Texas 77555-0635
Phone: (409) 772-8085
FAX: (409) 772-8025
Proteomics Bioinformatics Core
As part of the Proteomics Section of the Biomolecular Resource Facility
and consistent with its mission, the goal of the Proteomics
Bioinformatics Core is to assist researchers at UTMB in their analyses
of gels and other proteomics separation technologies.
- The Protein Bioinformatics Core provides informatics tools,
including statistical tools, for the analysis of 1D and 2D
electrophoresis gels utilizing a software suite from Totallab (formerly
Nonlinear Dynamics) and sample tracking, data management and access
tools, using software from GenoLogics (Proteus LIMS). The BRF is a major
Nonlinear and GenoLogics partner and collaborates with these
manufacturers in the development of their software.
- The Bioinformatics Core interacts extensively with other BRF
Cores, and UTMB Centers and Departments and has integrated the
Progenesis software into high-end user groups. For example, the BRF has
installed the Editor Progenesis software at a number of sites across the
- Group training sessions are scheduled as needed, typically every six months.
Mass Spectrometry Core
The aim of the UTMB Mass Spectrometry Core (MSC) is to assemble
state-of-the-art instrumentation and highly skilled technical staff to
provide experimental capabilities required to support a broad range of
molecular-level research. Mass spectrometry (MS) plays an increasingly
important role in molecular-level research, and it is central to ‘omics’
research. The MSC is well-equiped to facilitate studies with a suite
of LC-MS systems including the ThermoScientific Fusion Orbitrap MS, the
Sciex 5600 TripleTOF, 6500 Qtrap, and 5800 MALDI TOF/TOF.
- Molecular weight determination of biomolecules such as lipids, metabolites, peptides and proteins.
- Protein identification from gel plugs or bands and solutions using
enzymatic digestion followed by MS or MS/MS using either MALDI or LCMS.
- Proteomics: identification of large numbers of proteins from complex
mixtures using LCMS. Can include quantitative measurements.
- Identification of post-translational modifications.
- Differential quantitation by label-free (DIA, DDA, or SWATH), iTRAQ,
TMT, or SILAC using the Thermo Orbitrap Fusion or Sciex 5600+ TripleTof.
- Quantitation of molecules using Selected Reaction Monitoring (SRM) or multi-reaction monitoring (MRM) on a 6500 QTrap.
- Advanced informatics from proteomics datasets: Beyond Mascot and Sequest output.