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UTMB CRC Microbiome and Bioinformatics Analysis

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The Microbiome and Bioinformatics Analysis Core performs 16S rRNA, 18S rRNA, Internal Transcribed Spacer, and amplicon sequencing of microbial, viral, human, and animal samples (including feces, tissues, and intestinal lavage material) as well as other services related to microbiome and metabarcoding studies. Samples can be delivered frozen, at room temperature in a preservative such as 95% ethanol, or as extracted DNA. Sequence data are provided as FASTQ files and count tables of taxa, genes, or features. Additionally, we are happy to collaborate on bioinformatics analysis of microbiome data in conjunction with other multi-omics datasets (e.g. clinical metadata,metabolomics).

Available Services:

  • Sequencing Services
    • 16S ribosomal RNA gene sequencing
    • 18S ribosomal RNA gene sequencing
    • Internal Transcribed Spacer sequencing
    • Custom amplicon sequencing
    • DNA extraction with bead beating
  • Bioinformatics analysis (on a collaborative basis)
    • Differential taxa abundance in multivariate models
    • Microbial co-occurrence networks
    • Microbial modules
    • Hierarchical clustering
    • Unsupervised classification
    • Supervised classification
    • Predicted metagenomics
    • Multi-omics integration
    • Pathway analysis
    • Superimposition of datasets

Equipment for Sequencing and Analysis

  • Illumina MiSeq
  • Oxford Nanopore MK1
  • High Performance Computing Servers (64 core, 512 GB RAM) as well as accessibility to Texas Advanced Computing Center
  • CLC Genomics Workbench
    • Variant Analysis
    • Transcriptomics Analysis
  • CLC Microbial Genomics
  • Ingenuity Pathway Analysis