Faculty bio page2
Alex Bukreyev

Alexander Bukreyev, PhDProfessor

Departments of Pathology and Microbiology & Immunology
Phone: (409) 772-2829
Lab: (409) 772-2831
Email: alexander.bukreyev@utmb.edu
Bukreyev Laboratory Page

Alexander Bukreyev, PhD

Our Lab focuses on investigation of the mechanisms of the high pathogenicity of viruses Ebola, Marburg and Lassa and development of vaccines and antibody treatments against these viruses. More recently we also started work with respiratory syncytial virus (RSV), Crimean-Congo hemorrhagic fever virus and hantaviruses Andes and Sin Nombre.

Our research includes, but not limited to, the following specific topics:

  • Development of vaccines and investigation of the mechanisms of their protective effects.
  • In-depth characterization of antibody responses to viral infections in humans.
  • Development of therapeutic human monoclonal antibody treatments and investigation of the mechanisms of their protective effects.
  • Investigation of the mechanisms of immunosuppression caused by these viruses.
  • Comparative immunology of bats as a reservoir of emerging viral infections.

To get insight into these scientific topics, we are using molecular tools, including reverse genetics (i.e. development of genetically modified filoviruses from the DNA-copies of their genomes and use of mini-genomes), immunological tools such as multi-parameter flow cytometry, single-cell sequencing, human immune cells and animal models. Our research includes experiments in BSL-2, BSL-3 and BSL-4 labs of the Galveston National Laboratory. Our research is supported by grants from NIH and DoD and by contracts.

Our collaborators include Dr. James Crowe, Jr. (Vanderbilt University), Dr. Erica Ollmann Saphire (La Jolla Institute for Immunology), Dr. Galit Alter (Ragon Institute), Dr. Thomas Geisbert (UTMB), Dr. Raul Andino (UCSF), Moderna, Inc. and other groups.

MS Pyatigorsk Pharmaceutical Institute, Pyatigorsk, Russia. Pharmacy 1984
PhD State Research Center of Virology and Biotechnology "Vector", Koltsovo, Russia. Molecular Biology 1993
2017-2021 Member of the NIH Vaccines Against Microbial Diseases (VMD) Study Section.
2014 Member of the Organizing Committee of the 6th International Symposium on Filoviruses, Galveston, Texas, March 30-April 2, 2014.
2013 Convener of the Viral Epidemiology Workshop, 32th Annual Meeting of American Society for Virology, The Pennsylvania State University, University Park, Pennsylvania. July 20-24, 2013.
2012

Convener of the Emerging Viruses Workshop, 31th Annual Meeting of American Society for Virology, University of Wisconsin, Madison, Wisconsin. July 21-25, 2012.

Member of the Filoviridae Study Group of the International Committee on Taxonomy of Viruses, 2012-present.
2011

Member of Global Viral Network, 2011-present.

Convener of the Filoviruses Workshop, 30th Annual Meeting of American Society for Virology, University of Minnesota, Minneapolis, Minnesota. July 16, 2011.

American Society for Virology Program Planning Committee, 2011-2013.
2010 Convener of the Filoviruses Workshop, 29th Annual Meeting of American Society for Virology, Montana State University, Bozeman, Montana. July 17, 2010.
2009 Convener of the Vaccine and Viral Vectors III Workshop, 28th Annual Meeting of American Society for Virology, University of British Columbia, Vancouver, Canada. July 14, 2009.
2009 (two awards); 2008 (two awards); 2007; 2006 National Institute of Allergy and Infectious Diseases Performance Award in Recognition of Special Achievement in Support of the Mission of the National Institute of Allergy and Infectious Diseases.
2005 The National Institutes of Health Merit Award in Recognition of Outstanding Contributions and Efforts in Support of NIAID Mission.
2005; 2004; 2003; 2002; 2001 National Institute of Allergy and Infectious Diseases Staff Recognition Awards in Recognition and Appreciation of Special Achievement.
2000 The National Institute of Health Fellows Award for Research Excellence. 2000 (competitive).
1999 Special Act of Service Award, National Institute of Allergy and Infectious Diseases in Recognition and Appreciation of Special Achievement.
1996 - 1994 Personal Russian State Scholarship for Young Outstanding Scientists. 1994-1996. Awarded by the Presidium of the Russian Academy of Sciences. 1994.
  • International Society for Vaccines, Member, 2020 – present.
  • American Society for Microbiology, Contributing Member, 2019 – present.
  • American Society for Virology, Full Member, 1996 - present.

Selected publications in peer-reviewed journals

N. M. Lubaki, P. Ilinykh, C. Pietzsch, B. Tigabu, A. N. Freiberg, R. A. Koup, and A. Bukreyev (2013). The lack of maturation of Ebola virus-infected dendritic cells results from the cooperative effect of at least two viral domains. J. Virol. 87(13):7471-7485. The article was chosen for UTMB press release on 05/02/13.

P. A. Ilinykh, B. Tigabu, A. Ivanov, T. Ammosova, Y. Obukhov, T. Garron, N. Kumari, D. Kovalskyy, M. O. Platonov, V. S. Naumchik, A. N. Freiberg, S. Nekhai, and A. Bukreyev (2014). Role of protein phosphatase 1 in dephosphorylation of Ebola virus VP30 and its targeting for the inhibition of viral transcription. J Biol. Chem. 289(33): 22723-38.

A. I. Flyak, P. A. Ilinykh, C. D. Murin, T. Garron, X. Shen, M. L. Fusco, T. Hashiguchi, Z. A. Bornholdt, J. C. Slaughter, G. Sapparapu, T. G. Ksiazek, A. B. Ward, E. O. Saphire, A. Bukreyev*, and J. E. Crowe, Jr.* (2015) Mechanism of human antibody-mediated neutralization of Marburg virus. Cell 160(5): 893-903. The article was chosen for UTMB and the Vanderbilt University press releases on 5/26/2015. *Co-senior authors.

P. Ilinykh, M. Lubaki, S. Widen, L. A. Renn, T. C. Theisen, N. R. Rabin, T. Wood, and A. Bukreyev (2015) Different temporal effects of Ebola virus VP35 and VP24 on global gene expression in human dendritic cells. J. Virol. 90(15):7567-83.

M. Meyer, T. Garron, N. M. Lubaki, C. E. Mire, K. A. Fenton, C. Klages, G. G. Olinger, T. W. Geisbert, P. L. Collins, and A. Bukreyev (2015) Aerosolized Ebola vaccine protects primates and elicits lung-resident T-cell responses. J. Clin. Invest. 125(8): 3241-55. The article was chosen for UTMB press release and NIH press release on 7/13/2015.

A. I. Flyak, X. Shen, C. D. Murin, H. L. Turner, M. L. Fusco, R. Lampley, N. Kose, P. A. Ilinykh, N. Kuzmina, A. Branchizio, H. King, L. Brown, C. Bryan, E. Davidson, B. J. Doranz, J. C. Slaughter, G. Sapparapu, C. Klages, T. G. Ksiazek, E. O. Saphire, A. B. Ward, A. Bukreyev,* and J. E. Crowe, Jr.* (2016) Cross-reactive and potent neutralizing antibody responses in human survivors of natural Ebolavirus infection. Cell 164(3): 392-405. The article was chosen for UTMB and the Vanderbilt University press releases on 1/21/2016, and was highlighted in Nature 529, 440. *Co-corresponding authors.

P. A. Ilinykh, X. Shen, A. I. Flyak, N. Kuzmina, T. G. Ksiazek, J. E. Crowe, Jr., and A. Bukreyev (2016) Chimeric filoviruses for identification and characterization of monoclonal antibodies. J. Virol. 90(8): 3890-901.

A. Rialdi, L. Campisi, N. Zhao, A. C. Lagda, C. Pietzsch, J. S. Ho, L. Martinez-Gil, R. Fenouil, X. Chen, M. Edwards, G. Metreveli, S. Jordan, Z. Peralta, C. Muniz-Fontela, N. Bouvier, M. Merad, J. Jin, M. Weirauch, S. Heinz, C. Benner, H. van Bakel, C. Basler, A. Garcia-Sastre, A. Bukreyev, and I. Marazzi. (2016) Topoisomerase 1 inhibition suppresses inflammatory genes and protects from death by inflammation. Science. 352 (6289): aad7993. The article was chosen for Icahn School of Medicine at Mount Sinai press releases on 4/28/2016.

N. M. Lubaki*, P. Younan*, R. I. Santos, M. Meyer, M. Iampietro, R. A. Koup, and A. Bukreyev. (2016). The Ebola interferon inhibiting domains attenuate and dysregulate cell-mediated immune responses. PLOS Pathogens 21(12):e1006031. The article was chosen for UTMB press releases on 12/01/2016. *Co-lead authors.

I. V. Kuzmin, T. M. Schwarz, P. A. Ilinykh, I. Jordan, T. G. Ksiazek, R. Sachidanandam, C. F. Basler, and A. Bukreyev (2017). Innate immune response of bat and human cells to filoviruses: commonalities and distinctions. J. Virol. 91(8):e02471-16.

P. Luthra, S. Aguirre, M. T. Sanchez, B. C. Yen, C. A. Pietzsch, B. Tigabu, L. Morlock, A. Garcia-Sastre, A. Sesma, N. Williams, A. Bukreyev, D. W. Leung, and C. Basler. (2017). Topoisomerase II inhibitors induce DNA Damage dependent interferon responses circumventing Ebola virus immune evasion. J Biol. Chem.. 8(2) e00368-17.

P. Younan, P. Ramanathan, J. Graber, F. Gusovsky, and A. Bukreyev. (2017). The TLR4 antagonist Eritoran protects mice from lethal filovirus challenge. J Biol. Chem. 8(2) e00226-17.

M. Iampietro*, P. Younan*, A. Nishida, M. Dutta, N. M. Lubaki, R. I. Santos, R. A. Koup, M. G. Katze, and A. Bukreyev. (2017). Ebola virus glycoprotein directly triggers T lymphocyte death despite of the lack of infection. PLOS Pathogens 13(5):e1006397. The article was chosen for UTMB press releases on 05/24/2017. *Co-lead authors.

P. Younan, M. Iampietro, A. Nishida, P. Ramanathan, R. I. Santos, M. Dutta, N. M. Lubaki, R. A. Koup, M. G. Katze, and A. Bukreyev. (2017). Ebola binding to Tim-1 on T-Lymphocytes induces a cytokine storm. J Biol. Chem. 8(5) e00845-17. The article was chosen for American Society of Microbiology and UTMB press releases on 09/26/2017.

L. B. King, M. L. Fusco, A. I. Flyak, P. A. Ilinykh, K. Huang, B. Gunn, R. N. Kirchdoerfer., K. M. Hastie, A. Sangha, J. Meiler, G. Alter, A. Bukreyev, J. E. Crowe, Jr., and E. Ollmann Saphire. (2018) Therapeutic human monoclonal antibody MR191against Marburg virus targets a conserved site to block receptor binding. Cell Host & Microbe 23(1):101-109.

M. Meyer, E. Huang, O. Yuzhakov, P. Ramanathan, G. Ciaramella, and A. Bukreyev. (2019) Modified mRNA-based vaccines elicit robust immune responses and protect guinea pigs from Ebola virus disease. J. Infect. Dis. 217(2):451-455.

M. Meyer, E. Huang, O. Yuzhakov, P. Ramanathan, G. Ciaramella, and A. Bukreyev (2018) Modified mRNA-based vaccines elicit robust immune responses and protect guinea pigs from Ebola virus disease. J. Infect. Dis. 217(2):451-455.

P. Younan, M. Iampietro and A. Bukreyev (2018). Disabling of lymphocyte immune response by Ebola Virus. PLOS Pathogens 14(4):e1006932.

E. O. Saphire, S. L. Schendel, M. L. Fusco, K. Gangavarapu, B. M. Gunn, A. Z. Wec, P. J. Halfmann, J. M. Brannan, A. S. Herbert, X. Qio et al. (2018). Systematic analysis of monoclonal antibodies against Ebola virus GP defines features that contribute to protection. Cell 174(4):938-952.

A. I. Flyak, N. Kuzmina, C. D. Murin, C. Bryan, E. Davidson, P. Gilchuk, C. P. Gulka, P. A. Ilinykh, X. Shen, P. Ramanathan, K. Huang, H. Turner, M. L. Fusco, R. Lampley, N. Kose, H. King, G. Sapparapu, B. J. Doranz, T. G. Ksiazek, D. W. Wright, E. Ollmann Saphire, A. B. Ward, A. Bukreyev*, and J. E. Crowe*, Jr. (2018). Neutralizing antibodies from human survivors target a conserved site in the ebolavirus glycoprotein HR2/MPER regions. *Co-corresponding author. Nature Microbiology 3(6):670-677.

P. Gilchuk, N. Kuzmina, P. A. Ilinykh, K. Huang, B. M. Gunn, A. Bryan, E. Davidson, B. J. Doranz, H. L. Turner, M. L. Fusco, M. S. Bramble, N. A. Hoff, E. Binshtein, N. Kose, A. I. Flyak,, R. Flinko, C. Orlandi, R. Carnahan, E. H. Parrish, A. M. Sevy, R. G. Bombardi, P. K. Singh, P. Mukadi, J. J. Muyembe-Tamfum, M. D. Ohi, E. Ollmann Saphire , G. K. Lewis, G. Alter, A. B. Ward, A. W. Rimoin, A. Bukreyev*, and J. E. Crowe, Jr*. (2018). Multifunctional pan-ebolavirus antibodies recognize a site of broad vulnerability on the ebolavirus glycoprotein. Immunity 49(2):363-374. *Co-corresponding author. The article was chosen for NIAID press releases on 07/17/2018.

M. Meyer, A. Yoshida, P. Ramanathan, E. Ollmann Saphire, P. Collins, J. E. Crowe Jr., S. Samal and A. Bukreyev (2018). Antibody repertoires to the same Ebola vaccine antigen are differentially affected by vaccine vectors. Cell Reports 24: 1816-1829.

N. A. Kuzmina, P. Younan, P. Gilchuk, R. I. Santos, A. I. Flyak, P. A. Ilinykh, K. Huang, N. M. Lubaki, P. Ramanathan, J. E. Crowe, Jr. and A. Bukreyev (2018). Antibody-Dependent Enhancement of Ebola Virus Infection by Human Antibodies Isolated from Survivors. Cell Reports 24: 1802-1815.

M. Iampietro, R. I. Santos, N. M. Lubaki and A. Bukreyev (2018). Ebola Virus Shed GP Triggers Differentiation, Infection and Death of Monocytes Through TLR4 Activation. J. Infect. Dis. 218(Suppl. 5): S327-S334.

T. Ammosova, C. A. Pietzsch, Y. Saygideğer, A. Ilatovsky, X. Lin, A. Ivanov, N. Kumari, M. Jerebtsova, A. Kulkarni, M. Petukhov, A. Üren, A. Bukreyev* and Sergei Nekhai* (2018). Protein phosphatase-1-targeting small molecule C31 inhibits Ebola virus replication. J. Infect. Dis. 218 (Suppl. 5): S627-S635. *Co-corresponding author.

P. A. Ilinykh, J. Graber, N. A. Kuzmina, K. Huang, T. G. Ksiazek, J. E. Crowe, Jr. and A. Bukreyev (2018). Ebolavirus chimerization for the development of a mouse model for screening of Bundibugyo-specific antibodies. J. Infect. Dis. 218 (Suppl. 5): S418-S422.

P. A. Ilinykh, R. I. Santos, B. M. Gunn, N. A. Kuzmina, X. Shen, K. Huang, P. Gilchuk, A. I. Flyak, P. Younan, G. Alter, J. E. Crowe, Jr., and A. Bukreyev (2018). Assymetric antiviral effects of ebolavirus antibodies targeting glycoprotein stem and glycan cap. PLOS Pathogens 14(8):e1007204.

P. Younan, M. Iampietro, R. I. Santos, P. Ramanathan, V. L. Popov and A. Bukreyev (2018). Role of TMEM16F in the incorporation phosphatidylserine into budding ebolavirus virions. J. Infect. Dis. 218 (Suppl. 5): S335-S345.

P. Younan, M. Iampietro, R. I. Santos, P. Ramanathan, V. L. Popov and A. Bukreyev (2018). Disruption of phosphatidylserine synthesis or trafficking reduces infectivity of Ebola virus. J. Infect. Dis. 218 (Suppl. 5): S475-S485.

B. Tigabu, P. Ramanathan, A. Ivanov, X. Lin, P. A. Ilinykh, C. S. Parry, A. N. Freiberg, S. Nekhai and A. Bukreyev (2018). Phosphorylated VP30 of Marburg Virus is a Repressor of Transcription. J. Virol. 92(21):e00426-18.

M. Meyer, D. C. Malherbe and A. Bukreyev (2018). Can Ebola virus vaccines have universal correlates of protection? Trends in Microbiology 27(1):8-16.

J. Fang, C. Pietzsch, P. Ramanathan, R. I. Santos, P. A. Ilinykh, M. A. Garcia-Blanco, A. Bukreyev and S. S. Bradrick (2018). Staufen1 interacts with multiple components of the Ebola virus ribonucleoprotein and enhances viral RNA synthesis. J Biol. Chem. 9(5): e01771-18.

J. B. Kimble, D. C. Malherbe, M. Meyer, B. M. Gunn, M. M. Karim, P. A. Ilinykh, M. Iampietro, K. S. Mohamed, S. Negi, P. Gilchuk, K. Huang, Y I. Wolf, W. Braun, J. E. Crowe Jr., G. Alter and A. Bukreyev (2019). Antibody-mediated protective mechanisms induced by a trivalent parainfluenza-vectored ebolavirus vaccine. J. Virol. 93(4): e01845-18.

B. R. West, A. Z. Wec, C. L. Moyer, M. L. Fusco, P. A. Ilinykh, K. Huang, A. S. Wirchnianski, R. M. James, A. S. Herbert, S. Hui, E. Goodwin, K. A. Howell, S. Kailasan, M. J. Aman, L. M. Walker, J. M. Dye, A. Bukreyev, K. Chandran and E. Ollmann Saphire (2019). Structural Basis of Broad Ebolavirus Neutralization by a Human Survivor Antibody. Nature Structural & Molecular Biology 26(3):204-212.

L. B. King, B. R. West, C. L. Moyer, P. Gilchuk, A. Flyak, P. A. Ilinykh, R. Bombardi, S. Hui, K. Huang, A. Bukreyev, J. E. Crowe, Jr. and E. Ollmann Saphire (2019). Cross-reactive, neutralizing human survivor monoclonal antibody BDBV223 targets the ebolavirus stalk. Nature Communications 10 (1):1788.

P. Younan, R. I. Santos, P. Ramanathan, M. Iampietro, A. Nishida, M. Dutta, T. Ammosova, M. Meyer, M. G. Katze, V. L. Popov, S. Nekhai and A. Bukreyev (2019). Ebola virus-mediated T-lymphocyte depletion is the result of an abortive infection. PLOS Pathogens 15(10):e1008068. This article was chosen for UTMB Press Release on October 24, 2019.

A. Ivanov, P. Ramanathan, C. Parry, P. A. Ilinykh, X. Lin, Y. Obukhov, T. Ammosova, G. K Amarasinghe, A. Bukreyev* and S. Nekhai* (2019). Global phosphoproteomic analysis of Ebola virions reveals a novel role for VP35 phosphorylation-dependent regulation of genome transcription Cellular and Molecular Life Sciences 77(13):2579-2603. *Co-corresponding author.

P. Gilchuk, D. Murin, J.C. Milligan, R.W. Cross, C.E. Mire, P.A. Ilinykh, K. Huang, N. Kuzmina, P.X. Altman, S. Hui, B.M. Gunn, A.L. Bryan, E. Davidson, B.J. Doranz, H.L. Turner, T. Alkutkar, R. Flinko, C. Orlandi, R. Carnahan, R. Nargi, R.G. Bombardi, M.E. Vodzak, S. Li, A. Okoli, M. Ibeawuchi, B. Ohiaeri, G.K. Lewis, G. Alter, A. Bukreyev, E. Ollmann Saphire, T.W. Geisbert, A.B. Ward, J.E. Crowe Jr. (2020). Analysis of therapeutic antibody cocktail reveals determinants of cooperative and broad Ebola virus neutralization. Immunity 52(2):388-403.

A. N. Prasad, A. J. Ronk, S. G. Widen, T. G. Wood, C. F. Basler, A. Bukreyev (2020). Ebola virus produces discrete small noncoding RNAs independently of the host microRNA pathway which lack RNA interference activity in bat and human cells. Journal of Virology 94(6): e01441-19.

P. A. Ilinykh, K. Huang , R. I. Santos, P. Gilchuk, B. M. Gunn, M. M. Karim, J. Liang, M. E. Fouch, E. Davidson, D. V. Parekh, J. B. Kimble, C. A. Pietzsch, M. Meyer, N. A. Kuzmina, L. Zeitlin, E. O. Saphire, G. Alter, J. E. Crowe* and A. Bukreyev* (2020). Non-neutralizing antibodies from a Marburg infection survivor mediate protection by Fc-effector functions and by enhancing efficacy of other antibodies. Cell Host & Microbe 27(6):976-991.e11. This article was chosen for UTMB Press Release on April 21, 2020. *Co-corresponding author.

Z. J. Whitfield, A. N. Prasad, A. J. Ronk, I. V. Kuzmin, Philipp A. Ilinykh, R. Andino* and A. Bukreyev* (2020). Species-specific evolution of Ebola virus during replication in human and bat cells. Cell Reports 32(7):108028. This article was chosen for UTMB Press Release on August 18, 2020. *Co-corresponding author.

D. C. Malherbe, A. Domi, M. J. Hauser, M. Meyer, B. M. Gunn, G. Alter, A. Bukreyev* and F. Guirakhoo* (2020). Modified vaccinia Ankara vaccine expressing Marburg virus-like particles protects guinea pigs from lethal Marburg virus challenge. NPJ Vaccines 5:78. *Co-corresponding author.

N. G. Bozhanova, A. K. Sangha, A. M. Sevy, P. Gilchuk, P. A. Ilinykh, A. Bukreyev, J. E. Crowe, Jr. and J. Meiler (2020). Discovery of Marburg Virus Neutralizing Antibodies from Virus-Naïve Human Antibody Variable Gene Repertoires Using Large-scale Structural Predictions. Proc. Natl. Acad. Sci. USA 117(49):31142-31148.

C. Atyeo, M. D. Slein, S. Fischinger, J. Burke, A. Schӓfer, S. R. Leist, N. A. Kuzmina, A. Honko, R. Johnson6, N. Storm, M. Bernett, P. Tong, T. Zuo, J. Lin, A. Zuiani, C. Linde, T. Suscovich, D. Wesemann, A. Griffiths, J. R. Desjarlais, B. D. Juelg, J. Goutsmit, A. Bukreyev, R. Baric and G. Alter (2020). Dissecting strategies to tune the therapeutic potential of SARS-CoV-2-specific monoclonal antibody CR3022. JCI Insight 6(1):e143129.

A. Ilinykh and A. Bukreyev. Antibody responses to filovirus infections in humans: Protective or not? Lancet Infectious Diseases THELANCETID-D-20-04567R2 in press.

Michelle Meyer, Yuan Wang, Darin Edwards, Gregory R. Smith, Aliza B. Rubenstein, Palaniappan Ramanathan, Chad E. Mire, Colette Pietzsch, Xi Chen, Yongchao Ge, Wan Sze Cheng, Carole Henry, Angela Woods, LingZhi Ma, Guillaume B. E. Stewart-Jones, Kevin W. Bock, Mahnaz Minai, Bianca M. Nagata, Sivakumar Periasamy, Pei-Yong Shi, Barney S. Graham, Ian N. Moore, Irene Ramos, Olga G. Troyanskaya, Elena Zaslavsky, Andrea Carfi*, Stuart C. Sealfon*, Alexander Bukreyev* (2021). mRNA-1273 efficacy in a severe COVID-19 model: attenuated activation of pulmonary immune cells after the challenge. bioRxiv. 2021 Jan 25:2021.01.25.428136. doi: 10.1101/2021.01.25.428136. Preprint. *Co-corresponding author.